DS Calorimetry

The Hoch Lab maintains a MicroCal VP Capillary Differential Scanning Calorimeter. Contact Dr. Jeff Hoch directly to arrange access [Phone: (860) 679-3566; Email: hoch@.uchc.edu].

Fees
   Internal users: a small fee (about $2 per hour) to cover maintenance costs.
   External academic users: $25 per hour.
   Commercial users: $50 per hour.

http://www.microcalorimetry.com

Overview

Differential scanning calorimetry (DSC) quantifies the heat absorbed by a macromolecule as it undergoes a conformational or structural change. DSC is used to study a wide range of thermal transitions in biological systems, to determine melting temperatures as well as thermodynamic parameters associated with these changes. Because proteins in aqueous solutions are in equilibrium between their folded and unfolded states, DSC allows measurement of the number of intermediate steps that occur during a protein's denaturation process.

DSC References